Standard No.:	DB37/T 3531-2019
Chinese Name:	羊奶中羊、大豆源性成分的定性检测方法 实时荧光PCR法
English Name:	Identification of caprine，sheep and glycine derived materials in goat or sheep milk Real-time fluorescent PCR method
Professional Classification:	X16 Milk and dairy products
Professional Classification:	DB Provincial Standard
ICS Classification:	67.100.01 Milk and milk products in general
Issued by:
Issued on:	2019-03-21
Implemented on:	2019-04-21
Status:	VALID
Language:	English
File Format:	PDF
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Price(USD):	please email coc@codeofchina.com for quotation
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前言 II
1 范围 1
2 规范性引用文件 1
3 术语和定义 1
4 原理 1
5 试剂和材料 1
5.1 试剂 2
5.2 材料 2
6 仪器设备 2
7 检测用引物和探针 3
8 试验步骤 3
8.1 取样 3
8.2 DNA提取 3
8.3 实时荧光PCR反应 3
9 试验数据处理 4
9.1 PCR有效性判定 4
9.2 检测结果分析和表述 4
10 检测过程中防止交叉污染的措施 4
附　录　A （规范性附录） 实时荧光定性PCR引物/探针序列、反应体系和结果判定 5
附　录　B （资料性附录） 目的基因扩增靶标参考序列 7