This standard specifies the determination method of sucralose in foods.
This standard is applicable to the determination of sucralose in foods.
2 Theory
Extract the sucralose in the specimen with methanol aqueous solution, remove protein and fat, then separate it with high performance liquid chromatograph and reversed-phase C18 chromatographic column after purifying and gathering it with solid-phase extraction column, detect it with evaporative light-scattering detector or refractive index detector, conduct qualitative analysis according to retention time, and quantify it by peak area.
3 Reagents and Materials
Note: Unless otherwise specified, analytically pure reagents and Grade 1 water (specified in GB/T 6682) are adopted for the purpose of this method.
