Standard No.:	GB 5009.211-2014
Chinese Name:	食品安全国家标准 食品中叶酸的测定
English Name:
Professional Classification:	X09
Professional Classification:	GB National Standard
Issued by:
Issued on:	2015-09-21
Implemented on:	2016-03-21
Status:	VALID
Replace:	GB/T5009.211-2008
Target Language:	English
File Format:	PDF
Word Count:	8000 words
Translation Price:	240 (USD)
Delivery:	via email in 1-3 business day

1 Scope

This standard specifies determination method of folates in foods.

This standard is applicable to determination of folates in foods.

2 Principle

Folate is a necessary nutrient for growth of Lactobacillus casei spp.rhamnosus (ATCC 7469). Under certain control condition, inoculate Lactobacillus casei spp.rhamnosus in culture solution containing sample solution; after a certain period of cultivation , determine light transmittance (or absorbance); then calculate the content of folate in the sample according to the standard curve of folate content and light transmittance (or absorbance).

3 Reagents and Materials

Note: unless otherwise specified, all reactants adopted for this method are analytically pure and water is Grade II water as specified in GB/T 6682.

3.1 Reagents

3.1.1 Hydrochloric acid (HCl).

3.1.2 Sodium hydroxide (NaOH).

3.1.3 Sodium chloride (NaCl).

3.1.4 Sodium phorhoate tribaric dodecahydrate (Na3PO4?12H2O).

3.1.5 Disodium hydrogen phosphate heptahydrate (Na2HPO4?7H2O).

3.1.6 Dipotassium phosphate (K2HPO4).

3.1.7 Monopotassium phosphate trihydrate (KH2PO4?3H2O).

3.1.8 Magnesium sulfate heptahydrate (MgSO4?7H2O).

3.1.9 Ferrous sulfate heptahydrate (FeSO4?7H2O).

3.1.10 Manganese sulfate monohydrate (MnSO4?H2O).

3.1.11 Sodium acetate trihydrate (CH3CooNa?3H2O).

3.1.12 Glucose (C6H12O6).

3.1.13 Ascorbic acid (C6H8O6).

3.1.14 Methylbenzene (C7H8).

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