This standard specifies the liquid chromatography for the determination of polycyclic aromatic hydrocarbons (naphthalene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indeno [1,2,3-c,d] pyrene, dibenzo[a,h] anthracene and benzo[g,h,i]perylene) in foods and the gas chromatography-mass spectrometry for the determination of polycyclic aromatic hydrocarbons (naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indeno[1,2,3-c,d]pyrene, dibenzo[a,h]anthracene and benzo [g,h,i]perylene) in foods.
This standard is applicable to the determination of polycyclic aromatic hydrocarbon content in foods.
Method I High Performance Liquid Chromatography
2 Theory
Extract polycyclic aromatic hydrocarbons in the specimen with organic solvent, concentrate the extract solution to nearly dry, dissolve it with solvent and purify the solution with PSA (N-propyl ethylene diamine) and C18 solid-phase extraction filler or with magnesium trisilicate solid-phase extraction column. Concentrate it to the scale, then separate it by high performance liquid chromatography, determine the fluorescence intensity of polycyclic aromatic hydrocarbons at different excitation wavelengths and emission wavelengths, and then quantify it through external standard method.
3 Regents and Materials
GB 5009.265-2016 The following standards are cited：
GB 5009.265-2016 Cited by the following standards：
