This part of GB/T 19495 specifies the instruments and apparatus, reagents and materials, detection procedures, quality control, contamination-proof measures and minimum limit of detection of methods related to the screening of transgenic components and strain detection in plants and their processed products by qualitative real-time polymerase chain reaction (PCR) methods.
This part is applicable to transgenic screening and detection of plants such as soybean, maize, rape, rice, cotton, potato, flax, beet, alfalfa, tomato, papaya, apple, endive, bentgrass, nicotiana tabacum, plum, muskmelon, wheat, eggplant and eucalyptus, and is also applicable to the detection for strain specificity of plants such as soybean, maize, rape, cotton, rice, potato, flax, sugar beet and papaya.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use—Specification and test methods
GB/T 19495.2 Detection of genetically modified organisms and derived products—General requirements for laboratories
GB/T 19495.3 Detection of genetically modified organisms and derived products—Nucleic acid extraction
GB/T 19495.7 Detection of genetically modified organisms and derived products—Methods for sampling and sample preparation
GB/T 27403 Criterion on quality control of laboratories—Molecular biological testing of food
3 Terms, definitions and abbreviations
3.1 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1.1
transgene
technique in which a functional DNA sequence possessed by or derived from a species is bioengineered to be transcribed or expressed in the species in order to obtain a new trait of the species
3.1.2
real-time PCR
real-time polymerase chain reaction method in which a polymerase chain reaction system needs to add a fluorescent group whose fluorescence signal monitoring the entire PCR process in real time, and the unknown template is quantitatively analyzed by a standard curve
Note: The strength of the fluorescent signal directly reflects the number of templates.
3.1.3
endogenous gene
gene with constant copies and no allele changes were detected in the tested species
Note: This gene can be used to determine species specificity.
3.1.4
exogenous gene
other biological genes transferred using bioengineering techniques
Note: After transferring to an exogenous gene, the biological variety exhibits new biological traits.
3.1.5
cycle threshold
the number of cycles experienced by the fluorescent signal in each reaction tube reaching a set threshold
GB/T 19495.4-2018 The following standards are cited：
GB/T 19495.4-2018 Cited by the following standards：
