This standard specifies the liquid chromatography method for the determination of natamycin in food.
This standard is applicable to the determination of natamycin in food.
The quantitative detection limit of this standard is 0.5mg/kg (solid and semi-solid sample) and 0.5mg/L (liquid sample).
2 Principle
After extracting the sample with methanol, purify it through removing the fat component in it by adding water and freezing or with centrifugal method, then determine with reversed-phase liquid chromatography - ultraviolet detector and quantify with external standard method.
3 Reagents and Materials
3.1 Water: deionized water or water with equivalent purity.
3.2 Methanol: analytically pure or chromatographically pure.
3.3 Natamycin: with purity ≥95%.
3.4 Standard stock solution: accurately weigh adequate amount of natamycin standard product (accurate to 0.1mg) and dissolve with methanol to prepare standard stock solution with concentration of 100μg/mL.
3.5 Glacial acetic acid: top grade pure.
3.6 0.45μm and 0.22μm syringe-driven filters (water-based).
GB/T 21915-2008 The following standards are cited：
