This standard specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).
For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.
Note: a suitable method involving a cold saponification procedure is described in Annex B for information only.
2 Normative references
The following normative documents contain provisions which, through reference in this standard, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections) to, or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. For undated references, the latest edition applies.
GB/T 15687 GB/T 15687 Animal and vegetable fats and oils - preparation of test sample (GB/T 15687-2008, ISO 661:2003, IDT)
3 Terms and definitions
For the purpose of this standard, the following terms and definitions apply.
3.1
tocol content
mass fraction of the individual tocols, determined using the method specified in this standard
Note: the content is expressed in milligrams per kilogram as a whole number.
