This part of GB/T 31270 specifies the basic requirements for materials, conditions, operation, quality control, data processing, test reports, etc. for honeybee acute oral/contact toxicity test.
This part applies to honeybee acute oral/contact toxicity test for the registration of chemical pesticides. It may be used as a reference for other types of pesticides.
This part does not apply to volatile and insoluble chemical pesticides.
2 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
2.1
median lethal dose
dose of the test substance, at which 50% of the test organisms will be killed in the acute contact/oral toxicity test, , expressed by LD50
Note: the unit is in μg a.i./bee.
2.2
test substance
substance to be tested
2.3
chemical pesticide
pesticide made from chemical substances through artificial synthesis, some of which are synthetic bionic pesticides made through imitation, structural transformation and innovation by taking the active substances in natural products as the matrix
Synonym: synthetic organic pesticide.
[Definition 2.3.1, NY/T 1667.1-2008]
2.4
technical material
final product consisting of the active ingredients and impurities obtained during the manufacturing process, which shall not contain visible foreign substances or any additives, and if necessary, a small amount of stabilizer may be added
[Definition 2.5.1, NY/T 1667.2-2008]
2.5
formulation product
product stable in use and made from technical materials (or technical concentrate) of pesticides and auxiliaries
[Definition 2.4, NY/T 1667.2-2008]
2.6
active ingredient; a.i.
biologically active ingredient (with a specific chemical structure) in pesticide products
[Definition 3.1, NY/T 1667.2-2008]
2.7
reference substances
chemical substance or mixture used in a test to confirm or deny certain characteristics of a test substance or to judge the effectiveness of a test system
3 Test overview
3.1 Method overview
The honeybee acute toxicity test may be carried out via acute oral toxicity test or acute contact toxicity test, the selection of which is subjected to the regulations for pesticide registration management and other regulations.
