This part of GB/T 31270 specifies the basic requirements for materials, conditions, operation, quality control, data processing, test reports, etc. for daphnia sp. acute immobilisation test.
This part applies to daphnia sp. acute immobilisation test for the registration of chemical pesticides. It may be used as reference for other types of pesticides.
This part does not apply to volatile and insoluble chemical pesticides.
2 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
2.1
median effective concentration
concentration of the test substance, at which 50% of the test organisms are immobilised in the acute immobilisation test, expressed by EC50
Note: the unit is mg a.i./L.
2.2
immobilisation
phenomenon that the daphnia is not able to swim (but weak movement of appendages is allowed) within 15s when gently shaking the test vessel
2.3
test substance
substance to be tested
2.4
chemical pesticide
pesticide made of chemical substances through artificial synthesis, for some, the active substances in natural products are used as the mother body, and then subjected to imitation, structural transformation and innovation, they are bionic synthetic pesticides
Synonym: synthetic organic pesticide.
[Definition 2.3.1, NY/T 1667.1-2008]
2.5
technical material
final product consisting of the active ingredients and impurities obtained during the manufacturing process, which shall not contain visible foreign substances or any additives, and if necessary, a small amount of stabilizer may be added
[Definition 2.5.1, NY/T 1667.2-2008]
2.6
formulation product
product stable in use and made of technical material (or technical concentrate) of pesticides and auxiliaries
[Definition 2.4, NY/T 1667.2-2008]
2.7
active ingredient; a.i.
specific chemical ingredient that is biologically active in a pesticide product
[Definition 3.1, NY/T 1667.2-2008]
2.8
reference substances
chemical substance or mixture used in a test to confirm or deny certain characteristics of a test substance or to judge the effectiveness of a test system
3 Test overview
Prepare a series of test liquids with different concentrations, then observe the toxicity symptoms and immobilisation of test daphnia for consecutive 48h after transferring them into the test liquids, and calculate the EC50 (48h) and 95% confidence limit.
