This part of GB/T 31270 specifies the basic requirements for materials, conditions, operation, quality control, data processing, test reports, etc. for acute toxicity test of chemical pesticides on trichogramma.
This part is applicable to trichogramma acute toxicity test for the registration of chemical pesticides. It may be used as reference for other types of pesticides.
This part is not applicable to volatile and insoluble chemical pesticides.
2 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
2.1
median lethal rate
application quantity of the test substance, at which 50% of the test organisms die in the acute toxicity test, expressed by LR50
Note: the unit is mg a.i./ cm2 , i.e., the amount of active ingredient of the test substance attached per unit area.
2.2
safety factor
ratio of the median lethal rate LR50 of trichogramma to the recommended field applied concentration of the test pesticide, which may be expressed by Equation (1):
Safety factor= (1)
2.3
test substance
substance to be tested
2.4
chemical pesticide
pesticide made of chemical substances through artificial synthesis, some of which are synthetic bionic pesticides made through imitation, structural transformation and innovation by taking the active substances in natural products as the matrix
Synonym: synthetic organic pesticide.
[Definition 2.3.1, NY/T 1667.1-2008]
2.5
technical material
final product consisting of the active ingredients and impurities obtained during the manufacturing process, which shall not contain visible foreign substances or any additives, and if necessary, a small amount of stabilizer may be added
[Definition 2.5.1, NY/T 1667.2-2008]
2.6
formulation product
product stable in use and made of technical material (or technical concentrate) of pesticides and auxiliaries
[Definition 2.4, NY/T 1667.2-2008]
2.7
active ingredient; a.i.
biologically active ingredient (with a specific chemical structure) in pesticide products
[Definition 3.1, NY/T 1667.2-2008]
3 Test overview
Prepare the test substance into a series of diluents of different concentrations by a solvent such as acetone, quantitatively add it into a finger-shaped tube to prepare a pesticide film tube, put the test trichogramma into it to crawl for 1h and then transfer the trichogramma into a finger-shaped tube without pesticide; check the survival of trichogramma 24h later. Obtain the LR50 value and 95% confidence limit of trichogramma against the pesticide.
