Standard No.:	GB/T 33392-2016
Chinese Name:	皮革和毛皮 化学试验 禁用偶氮染料中4-氨基偶氮苯的测定
English Name:	Leather and fur—Chemical tests—Determination of 4-aminoazobenzene derived from azo colorants
Professional Classification:	Y46 Furs and leathers
ICS Classification:	59.140.30 Leather and furs
Issued by:
Issued on:	2016-12-30
Implemented on:	2017-07-01
Status:	VALID
Language:	English
File Format:	PDF
Word Count:	8000 words
Price(USD):	240 (USD)
Delivery:	via email in 1-3 business day

1 Scope

This standard specifies the detection method of gas chromatography-mass spectrometry and high performance liquid chromatography for 4-aminoazobenzene (CAS No.: 60-09-3) derived from some azo colorants and existed in free state.

This standard is applicable to various leathers, furs and their products.

2 Normative References

The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.

GB/T 6682-2008 Water for Analytical Laboratory Use - Specification and Test Methods (ISO 3696:1987, MOD)
QB/T 1267 Fur - Chemical, Physical and Mechanical and Fastness Tests - Sampling Location
QB/T 1272 Fur - Preparation of Chemical Test Samples
QB/T 2706 Leather - Chemical, Physical and Mechanical and Fastness Tests - Sampling Location (QB/T 2706-2005, ISO 2418:2002, MOD)
QB/T 2716 Leather - Preparation of Chemical Test Samples (QB/T 2716-2005, ISO 4044:1997, MOD)

3 Principle

After degreasing, the specimen is treated with sodium dithionite in an alkaline medium at 40℃ in a closed vessel. 4-aminoazobenzene obtained by reductive cleavage in the process is extracted with tert-butyl methyl ether. The determination of 4-aminoazobenzene is performed using gas chromatography with a mass-selective detector (GC-MS) or high-performance liquid chromatography with a diode array detector (HPLC/DAD).