General Administration of Quality Supervision, Inspection and Quarantine
Issued on:
2016-12-12
Implemented on:
2017-07-01
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Textiles for import and export—Determination of glyoxal, formaldehyde and glutaraldehyde—
High performance liquid chromatography
1 Scope
This standard specifies high performance liquid chromatography (HPLC) for determination of free and hydrolyzed glyoxal, formaldehyde and glutaraldehyde in textiles.
This standard is applicable to the determination of free and hydrolyzed glyoxal, formaldehyde and glutaraldehyde content in various textile materials and their products.
2 Normative reference
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use—Specification and test methods
3 Principle
The specimen is extracted with water, and the aldehyde compounds in the extraction solution act with 2,4-dinitrophenylhydrazine under acidic conditions to produce 2,4-dinitrophenylhydrazone accordingly, which then is separated by using reversed-phase liquid chromatography column, and determined by high performance liquid chromatography equipped with secondary array tube detector or ultraviolet detector, and finally be quantified with external standard method.
4 Reagents and materials
Unless otherwise specified, the reagents used are analytical grade, and the water used is Grade I water conforming to GB/T 6682.
4.1 Acetonitrile: chromatographically pure.
4.2 Glacial acetic acid.
4.3 2,4-dinitrophenylhydrazine (DNPH)
4.4 2,4-dinitrophenylhydrazine solution: weigh 0.07 g of 2,4-dinitrophenylhydrazine (4.3) into a 100 mL beaker, add about 80 mL of acetonitrile and 0.5 mL of glacial acetic acid, and stir until dinitrophenylhydrazine is completely dissolved, then immediately transfer it to a 100 mL brown volumetric flask, dilute to the scale with acetonitrile, and shake well.
4.5 Formaldehyde standard aqueous solution: with a concentration of 1,000 mg/L.
4.6 Glyoxal standard aqueous solution: with a concentration of 1,000 mg/L.
4.7 Glutaraldehyde standard aqueous solution: with a concentration of 1,000 mg/L.
