Standard No.:	GB/T 5009.179-2003
Chinese Name:	火腿中三甲胺氮的测定
English Name:	Determination of trimethylamine nitrogen in ham
Professional Classification:	C53 Food sanitation
Professional Classification:	GB National Standard
ICS Classification:	67.040
Issued by:
Issued on:	2003-08-11
Implemented on:	2004-01-01
Status:	ABOLISHED
Replace By:	GB5009.179-2016
Replace By Date:
Language:	English
File Format:	PDF
Word Count:	2000 words
Price(USD):	60 (USD)
Delivery:	via email in 1-3 business day

1 Scope

This standard specifies determination of trimethylamine nitrogen in ham.
This standard is applicable to determination of trimethylamine nitrogen in ham.

2 Principle

Trimethylamine [(CH3)3N], as a volatile alkali nitrogenous substance, is produced when fish flesh reduces trimethylamine oxide [(CH3)3NO] due to the action of bacteria in the decay process. It is extracted into anhydrous toluene to generate yellow picric acid trimethylamine salt under the action of picric acid. Then make it compare colour with standard tube. The content of trimethylamine nitrogen in the sample can be determined.

3 Reagent

3.1 20% trichloroacetic acid solution.