This standard specifies the method for determination of chloropropanols in foods.
Method I in this standard is applicable to the determination of 3-monochloro-1,2-propanediol (3-MCPD) in hydrolyzed vegetable protein liquid, flavorings, sausages, cheese, fish, flour, starch, cereals and breads. Method II in this standard is applicable to the determination of monochloro substituted 3-monochloro-1,2-propanediol (3-MCPD) and 2-monochloro-1,3-propanediol (2-MCPD) and dichloro substituted 1,3-dichloro-2-propanol (1,3-DCP) and 2,3-monochloro-1-propanol (2,3-DCP) in flavorings like soy sauce, vinegar, chicken powder and oyster sauce, hydrolyzed vegetable protein liquid, sausages, instant noodle seasonings. Method III in this standard is applicable to the determination of 3-MCPD, 1,3-DCP and 2,3-DCP in soy sauce, vinegar, chicken powder, hydrolyzed vegetable protein liquid and flavorings.
In this standard, determination limit and linear range for Method I are respectively 5μg/kg and 0.005ng ~ 0.600ng. Detection limit and quantitative limit for components of chloropropanol in Method II are respectively 2.0μg/kg and 5.0μg/kg, and the linear range is 0.020ng ~ 0.500ng. For Method III, quantitative limits for 3-MCPD, 1,3-DCP and 2,3-DCP are respectively 10.0μg/kg, 5.0μg/kg and 3.0μg/kg, the linear range for 3-MCPD is 20μg/kg ~ 1375μg/kg and that for both 1,3-DCP and 2,3-DCP is 2.5μg/kg ~ 90μg/kg.
Method I Determination of 3-Monochloro-1,2-Propanediol in Foods
2 Principle
Isotope dilution technology is adopted and d5-3-monochloro-1,2-propanediol (d5-3-MCPD) is adopted for internal standard quantitative analysis. Add internal standard solution into the sample, adopt kieselguhr (ExtrelutTM20) as the adsorbent to perform column chromatography separation, elute nonpolar lipid components in the sample by way of normal hexane-ethyl ether (9+1) and 3-MCPD in the sample by way of ethyl ether, and adopt heptafluorobutyryl imidazole (HFBI) solution as the derivatization reagent. Perform quantitative analysis by way of selected ion monitoring (SIM) in mass spectrum scanning mode, and quantify with internal standard method.
3 Reagents and Materials
GB/T 5009.191-2006 The following standards are cited：
GB/T 5009.191-2006 Cited by the following standards：
