Standard No.:	GB/T 5009.194-2003
Chinese Name:	保健食品中免疫球蛋白IgG的测定
English Name:	Determination of immunoglobulin iin health foods
Professional Classification:	C53 Food sanitation
Professional Classification:	GB National Standard
ICS Classification:	67.040
Issued by:
Issued on:	2003-08-11
Implemented on:	2004-01-01
Status:	VALID
Language:	English
File Format:	PDF
Word Count:	2500 words
Price(USD):	75 (USD)
Delivery:	via email in 1-3 business day

1 Scope

This Standard specifies the determination method of immunoglobulin in health food.
This Standard applies to the determination of immunoglobulin in tablet-type, capsule-type and powder-type health food.
The detection limit of this method: where the sampling quantity is 0.1g, which is diluted to 25mL, and where the injection quantity is 20μL, the detection concentration is 0.5mg/mL.

2 Principle

According to the principle of high-performance affinity chromatography, under the condition of phosphate buffer solution, immunoglobulin connects to aglucone; under the condition of hydrochloric acid glycine (pH2.5), the immunoglobulin is eluted.