Standard No.:	GB/T 5009.82-2003
Chinese Name:	食品中维生素A和维生素E的测定
English Name:	Determination of retinol and tocopherol in foods
Professional Classification:	C53 Food sanitation
Professional Classification:	GB National Standard
ICS Classification:	67.040
Issued by:
Issued on:	2003-08-11
Implemented on:	2004-01-01
Status:	ABOLISHED
Replace By:	GB5009.82-2016
Replace By Date:
Language:	English
File Format:	PDF
Word Count:	4000 words
Price(USD):	120 (USD)
Delivery:	via email in 1-3 business day

1 Scope

This standard specifies the method for the determination of retinol and tocopherol in foods.
This standard is applicable to the determination of retinol and tocopherol in foods.
The detection limits of this standard are: VA: 0.8ng; α-E: 91.8ng; γ-E: 36.6ng; δ-E: 20.6ng.

Method I High Performance Liquid Chromatography

2 Principle

After saponification treatment, retinol and tocopherol in the specimen shall be extracted from the non-saponifiable part into the organic solvent, separated with high performance liquid chromatography C18 reversed phase column, detected by UV detector and then determined quantitatively with internal standard method.